Fluo
Images and results with Fluorescence applications
Fluorescence Station[img alt="" src="http://www.exploranova.com/wp-content/flagallery/fluo/thumbs/thumbs_tuj0006_deconvol.jpg"]
Deconvolution[img alt="" src="http://www.exploranova.com/wp-content/flagallery/fluo/thumbs/thumbs_fluocells.jpg"]
Fluorescent Cells
Fluo Suite was a complete system aimed at fluorescence microscopy which has now been incorporated into the Morpho Strider – general image analysis application. It was developed in collaboration with cutting-edge research teams in the fluorescence microscopy area..
Examples
Extended Depth Focus from a Z Image Stack:
Cell culture – Before and after deblurring:
Histology image stack – Before and after deblurring:
Key Features
2D Deconvolution
- Deblurring methods: No Neighbor;
- Restoration methods: Lucy-Richardson, Gold-Meinel;
- Blind Deconvolution
3D Deconvolution
- Pearson’s coefficient;
- Manders;
- Overlap;
- Colocalization m1 m2;
- Icorr – nMDP (normalized Mean Deviation Product);
- Scatterplot;
3D visualization
- Volume rendering for 3D stack of images (free handling : translation, rotation, magnification) to obtain a better visualization.
- 3D Image Section X-Z et Y-Z to verify the colocalization
- Export of video sequences (AVI)
Morphometric & Densitometric Analysis
- Morphometry: surface, diameter, perimeter,…
- Densitometry: sum of intensity by channel, profiles, histogram of grey levels,…
- Nearest neighbors & maximum likelihood
Structured light acquisition
Structured light acquisition with OptiGrid provides optical sectioning with unprecedented simplicity and light efficiency, while maintaining the optical integrity and image quality of its host microscope. OptiGrid offers confocal fluorescence image sectioning and multiple fluorescence Image sectioning and software for extended depth of focus, 3D and surface reconstruction.
Compatible with the Full Spectrum of Fluorescence Dyes
Angstrom from Quorum Technologies
This product was designed to circumvent common engineering limitations and restrictions found in the illumination pathways of microscope stands available in today’s market. The Angstrom is an optical projection device. The system is modular in nature and can be used for several microscope applications, the most common of which are associated with fluorescence illumination.
l-DOPA Impairs Proteasome Activity in Parkinsonism through D1 Dopamine Receptor
C Caillava, R Vandenbosch… – The Journal of Cell …, 2011 – jcb.rupress.org
… 2 ). In the anterior subventricular zone (six 12-µm sagittal sections), results were expressed as the number of cells per surface (mm 2 ). Images were acquired with a 40× objective lens using a fluorescent microscope (DBM; Leica) and image acquisition software (Explora Nova). …
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Ectopic expression of polysialylated neural cell adhesion molecule in adult macaque Schwann cells promotes their migration and remyelination potential in the central … [HTML] from oxfordjournals.org
C Bachelin, V Zujovic, D Buchet, J Mallet… – Brain, 2010 – Oxford Univ Press
… Coverslips were mounted with fluoromount G (Southern Biotechnology, Alabama, USA) and evaluated using a DBM Leica fluorescence microscope and Explora Nova (La Rochelle, France) image acquisition software. In vitro statistical analysis. …
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A Gaillard, M Decressac, I Frappé… – Neurobiology of …, 2009 – Elsevier
The main transplantation strategy in Parkinson’s disease has been to place dopaminergic grafts not in their ontogenic site, the substantia nigra, but in their target area, the striatum with contrasting results. Here we have used green fluorescent protein transgenic…
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Parkin deficiency delays motor decline and disease manifestation in a mouse model of synucleinopathy [HTML] from plos.org
M Fournier, J Vitte, J Garrigue, D Langui, JP Dullin… – PloS one, 2009 – dx.plos.org
The same procedure was used twice sequentially for double stainings. Fluorescent images were acquired with an epifluorescensce microscope (Axioplan 2, Zeiss, Germany) equipped with the FluoUp image analysis system (Explora Nova, La Rochelle, France).
[PDF] from epfl.chM Fournier, J Vitte, J Garrigue, D Langui… – 2009 – infoscience.epfl.ch
Page 1. Parkin Deficiency Delays Motor Decline and Disease Manifestation in a Mouse
Model of Synucleinopathy Margot Fournier1,2,3, Je´re´mie Vitte1,2,3, Jéroˆme
Garrigue1,2,3, Dominique Langui1,2,3, Jean-Philippe Dullin1 …
A Colin, M Faideau, N Dufour, G Auregan, R Hassig… – Glia, 2009 – Wiley Online Library
… images (six images per section, three sections, three animals) using a 63× objective on a Zeiss Axioplan2 imaging microscope equipped with automated motorized stage and an image acquisition and analysis system (FluoUp and Mercator software, Explora Nova, La Rochelle …
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K Nouette-Gaulain, N Bellance, B Prévost… – …, 2009 – journals.lww.com
Background: Local anesthetics offer the benefits of extended analgesia with greater patient satisfaction and faster rehabilitation compared with intravenous morphine. These benefits, however, can be offset by adverse iatrogenic muscle pain caused by bupivacaine. Here, the …
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Normal aging modulates the neurotoxicity of mutant huntingtin [HTML] from plos.org
E Diguet, F Petit, C Escartin, K Cambon, N Bizat… – PloS one, 2009 – dx.plos.org
… enzymatic activity and β-Gal immunofluorescence were scanned at 10× objective using a Zeiss Axioplan 2 Imaging microscope equipped with a motorized stage and an image acquisition and analysis system image (FluoUp and Mercator softwares, Explora Nova, La Rochelle …
Cited by 4 – Related articles – Cached – All 6 versions
Comprehensive Invited Review [PDF] from oroboros.at
G BENARD… – ANTIOXIDANTS & REDOX SIGNALING, 2008 – oroboros.at
… (B) Fluo’up software (Explora Nova, La Rochelle, France) was used to calculate the skeleton of the mitochondrial network and analyze the degree of branching, as well as the length of the tubules and the num- ber of fragments. …
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