Morpho Strider is an almost all purpose image analysis software that includes :
- Data Management
- Automation
- Acquisition (Brightfield & Fluorescence)
- Processing & Extraction
- Deconvolution
- Fluorescence dedicated functions
- 3D Reconstruction
Fluo
Images and results with Fluorescence applications
Fluorescence Station[img alt="" src="http://www.exploranova.com/wp-content/flagallery/fluo/thumbs/thumbs_tuj0006_deconvol.jpg"]
Deconvolution[img alt="" src="http://www.exploranova.com/wp-content/flagallery/fluo/thumbs/thumbs_fluocells.jpg"]
Fluorescent Cells
Fluo Suite was a complete system aimed at fluorescence microscopy which has now been incorporated into the Morpho Strider – general image analysis application. It was developed in collaboration with cutting-edge research teams in the fluorescence microscopy area..
Examples
Extended Depth Focus from a Z Image Stack:
Cell culture – Before and after deblurring:
Histology image stack – Before and after deblurring:
Key Features
2D Deconvolution
- Deblurring methods: No Neighbor;
- Restoration methods: Lucy-Richardson, Gold-Meinel;
- Blind Deconvolution
3D Deconvolution
- Pearson’s coefficient;
- Manders;
- Overlap;
- Colocalization m1 m2;
- Icorr – nMDP (normalized Mean Deviation Product);
- Scatterplot;
3D visualization
- Volume rendering for 3D stack of images (free handling : translation, rotation, magnification) to obtain a better visualization.
- 3D Image Section X-Z et Y-Z to verify the colocalization
- Export of video sequences (AVI)
Morphometric & Densitometric Analysis
- Morphometry: surface, diameter, perimeter,…
- Densitometry: sum of intensity by channel, profiles, histogram of grey levels,…
- Nearest neighbors & maximum likelihood
Structured light acquisition
Structured light acquisition with OptiGrid provides optical sectioning with unprecedented simplicity and light efficiency, while maintaining the optical integrity and image quality of its host microscope. OptiGrid offers confocal fluorescence image sectioning and multiple fluorescence Image sectioning and software for extended depth of focus, 3D and surface reconstruction.
Compatible with the Full Spectrum of Fluorescence Dyes
Angstrom from Quorum Technologies
This product was designed to circumvent common engineering limitations and restrictions found in the illumination pathways of microscope stands available in today’s market. The Angstrom is an optical projection device. The system is modular in nature and can be used for several microscope applications, the most common of which are associated with fluorescence illumination.
A Courtin, L Communal, M Vilasco… – … Cancer Research and …, 2011 – Springer
… antibody (1:5000) (Santa Cruz, Heidelberg, Germany). Quantification of the relative expression of GR was performed using Explora- Nova Morpho Expert software. Reporter enzyme assays Cells were transfected with MMTV-LUC …
J Hémar, T Sauvigné, AG Bodard… – Médecine Buccale …, 2011 – mbcb-journal.org
… each microradiograph. The degree of mineralization of bone (DMB) was quantified using automatic software for gray level analysis (Morpho Expert and Mineralization, Explora Nova, La Rochelle, France). Microscopic images …
D Pillon, V Cadiou, L Angulo… – Brain Research, 2011 – Elsevier
… Embryos and placenta were weighted on a balance (Sartorius TE124S 0.1 mg precision). Crown-rump length and head diameter were measured using an image analysis system (Visiolab, Explora Nova, La Rochelle, France). Embryos were staged using the Theiler atlas. …
C Goizet, C Depienne, G Benard… – Human …, 2011 – Wiley Online Library
… mitochondrial tubule length was performed using a double-blinded approach. The length of the mitochondrial tubules was evaluated with the morphometric software (Morpho.Pro; Explora Nova, France). This allowed the region …
[HTML] à partir de plos.orgA Bencsik… – PloS one, 2011 – dx.plos.org
… eosin (HE) respectively. Lesion profiles were established according to Fraser’s lesion profile [31] by quantification using a computer-assisted method (Morpho Expert software, Explora Nova, La Rochelle, France) [32]. Brain slices …
… microdamage, collagen cross‐links, degree of mineralization, age, and architecture on compressive mechanical properties of elderly human vertebral trabecular bone [PDF] from inserm.fr
H Follet, S Viguet‐Carrin… – Journal of …, 2011 – Wiley Online Library
… 11 Three sections per specimen—bulk stained with xylenol orange—were measured using fluorescence (excitation/emission wavelength of 440–570/610 nm) microscopy at ×200 magnification and morphometry software (Bone Morpho; Explora Nova, La Rochelle, France). …
Cited by 1 – Related articles – All 9 versions
B Burt-Pichat, H Follet, G Toulemonde, M Arlot… – Journal of Bone and … – Springer
… magnification and using morphometry software (Bone Morpho; Explora Nova, La Rochelle, France). …visible but tetracycline labeling (double labeling) faded and the bone area was … cracks were visible but double labeling was faint (histomorphometry measurement unavailable). …
Related articles
Emergence of Classical BSE Strain Properties during Serial Passages of H-BSE in Wild-Type Mice [HTML] from plos.org
T Baron, J Vulin, AG Biacabe, L Lakhdar… – PLoS One, 2011 – dx.plos.org
… Finally, the slides were counterstained with aqueous hematoxylin, dehydrated, mounted using Eukitt and observed under a light microscope BX51 (Olympus, France) coupled to an image analysis workstation (MorphoExpert software, Explora Nova, La Rochelle, France). …
Cited by 2 – Related articles – Cached – All 5 versions
XPC silencing in normal human keratinocytes triggers metabolic alterations that drive the formation of squamous cell carcinomas [HTML] from nih.gov
HR Rezvani, AL Kim, R Rossignol… – The Journal of …, 2011 – ncbi.nlm.nih.gov
Doublecortin-positive cells in the adult primate cerebral cortex and possible role in brain plasticity and development [PDF] from rero.ch
J Bloch, M Kaeser, Y Sadeghi… – The Journal of …, 2011 – Wiley Online Library
… magnification images were observed under an epifluorescent microscope (IX81 Olympus) equipped with FITC, Cy3, Dapi, and infrared 680 nm filters for multichannel images that were acquired with two image analysis programs (CellM, Olympus or MorphoPro, Explora-Nova). …
Related articles – All 3 versions
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