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Fluo Suite is a full system aimed at fluorescence microscopy. Another user friendly product to ensure top quality images and results.
The suite consists of: Fluo'Up and Fluo'D3
Developed in collaboration with cutting-edge research teams in the fluorescence microscopy area, this system was designed to meet all your requirements. The system can be customized and this allows you to optimize all the acquisition parameters, so that they match the equipment used and the biological application. It also facilitates darkroom work.
Fluo'Up
Fluo'Up has an interface that has been specially designed to integrate the specific constraints of fluorescence imaging: the result is an essentially simple interface, smooth workflow and more.
The module also integrates the full set of controls for microscope functions: objectivs, motirized XY stages, Z motor, filter whees, shutters, etc.
High resolution monochrome or color digital cameras have been carefully chosen according to the prefered sensitivity and wavelengths.
During acquisition the user is allowed to choose the filters to be used (filters are listed by color), control the microscope, adjust the acquisition conditions (duration, gain, etc.) and acquire images. Six acquisition protocols (RGB or Wavelength) enable you to fine-tune the acquisition quality. A final image is created by adjusting the color balance.
For each acquisition protocol th user can remove the background using sophisticated image processing algorithms.
Tme-lapse and Z-stacked images can be acquired according to user-defined time frequency or a height along the Z-axis, thus generating an image stack that can subsequently be analyzed and compared.
Fluo'D3
Fluo'D3 corrects optical blurring and removes out-of-focus haze from 3D images.
It analyzes images using a large range of deblurring algorithms:
the constrained-iterative-program;
the nearest-neighbor and;
the no-neighbor program and more.
As a result, the user is able to see a 3D stack of images:
topographic;
surface and;
XZ and YZ profiles.
Deblurring:
The deblurring algorithms, as mentioned above, enable an increase in contrast for the observed image by reducing the weight of contributions derived from non-focal planes.
It could be applied on individual images or on a stack of images (3D deconvolution).
Methods of deblurring require a precise knowledge of the function of apparatus (PSF), which characterizes the microscope, in various experimental contexts and which defines the degradations introduced by the varios elements of the optical system.
Several approaches are possible to obtain this function.
In a first approach, of experimental type, the PSF of the instrument is characterized thanks to the visualization of preparations of latex microbeads gauged coated with chromophoric used in the studies, which each one are regarded as a point source and whose image makes it possible to obtain the PSF.
A second approach consists of calculating a theoretical PSF starting from a mathematical model which represents optically the system unit/sample (Agard 89).
A third approach consists of carrying out convolution via a "blind study", i.e. the data itself . The great advantage of this last type of method is that it can obtain, not only the restored images but also the PSF which corresponds to the experiment.
3D vizualisation:
With images being enhanced, sharpened and reconstructed,researchers can see the detail and create new perpesctives of their samples that they could not otherwise do using only the oculars of their microscope.
Using a stack of planes from a through-focus set of images (Z-series), the user configures the 3D Reconstruction dialog box to set the angle, orientation and Z-axis distance.
An AVI Movie can be recorded and exported in a predefined rotation.
XZ and YZ Profiles:
The View Orthogonal Planes command is an interactive image viewer which lets you examine cross-sectional slices through the image volume in the XZ, YZ, and XY planes. This helps you to view three-dimensional structural information about a specimen.
Colocalization:
A specialized module gives the user the capability of calculating the colocalization coefficients of different staining (Pearson, Manders, k1, k2, m1, m2...).The user can easily study the area in relation to a part of the scatterplot. An image of precise colocalization is then available.
Batch Processing:
This is done by a specalized module which can pause on command and is very user friendly.
Extended Depth Focus from a Z Image Stack:
EXAMPLE 1
Cell culture: Before deblurring:
After deblurring:
EXAMPLE 2
Histology image stack - Before deblurring:
After deblurring
KEY FEATURES
2D Deconvolution
Deblurring methods: No Neighbor
Restoration methods: Lucy-Richardson, Gold-Meinel.
Blind Deconvolution
3D Deconvolution
Pearson's coefficient
Manders
Overlap
Colocalization m1 m2
Icorr - nMDP (normalized Mean Deviation Product)
Scatterplot
3D visualization
Volume rendering for 3D stack of images (free handling : translation, rotation, magnification) to obtain a better visualization.
3D Image Section X-Z et Y-Z to verify the colocalization
Export of Video sequences (AVI)
Morphometric & Densitometric Analysis
Morphometric parameters: Surface, Diameter, Perimeter,...
Densitometric parameters: sum of Intensity by channel, profiles, Histogram of Grey Levels,...
Nearest Neighbors & Maximum Likelihood
The Fluo Suite sofware is capable of treating images from various sources eg. Leica - LIF format, TIF, JPG or Metamorph STK format.
Structured light acquisition
Designed for use with all of today's Florescence and reflected light microscopes, OptiGrid provides optical sectioning with unprecedented simplicity and light efficiency, while maintaining the optical integrity and image quality of its host microscope.
Truly innovative technology and a clean, uncomplicated design translate into a significantly lower entry cost than alternative confocal technologies. With OptiGrid, research quality confocal imaging is accessible to most life sciences and materials laboratories.
Compatible with the Full Spectrum of Fluorescing Dyes
OptiGrid offers confocal fluorescence image sectioning and multiple fluorescence Image sectioning and software for extended depth of focus, 3D and surface reconstruction. For the first time, the scientific community will be able to capture confocal-fluorescent images that have been previously unobtainable ... including those using GFP Dyes.
Because OptiGrid utilises conventional light, it illuminates specimens in the same manner as standard fluorescence microscopes, affording universal compatibility with the full-spectrum of fluorescent dyes. This also minimises fluorescent dye bleaching and photo-degradation inherent in laser confocal imaging.
Confocal Microscopy is now redefined with OptiGrid. Optigrid makes use of a unique Grid Projection technology to deliver the full capability of confocal microscopy without the use of lasers, pin-holes or de-convolution software. Your existing Fluorescence or reflected light microscope now may be used to obtain high quality optical sections with ease.
The acquired sections are then reconstructed as 3D or extended depth of focus images. Optigrid covers many Fluorescence and reflected light applications.The cost of a confocal microscope is now much reduced, with a highly competitive purchase price and low cost maintenance and little downtime.
New product: Angstrom from Quorum Technologies
The Quorum angstrom is a unique product designed specifically to circumvent common engineering limitations and restrictions found in the illumination pathways of microscope stands available in today's market. The Angstrom is an engineered optical projection device with enhanced optical components. The system is modular in nature and can be used for several microscope applications, the most common of which are associated with fluorescence illumination. The Angstrom can be adapted to any microscope that has a photo-port or, alternatively, optical adapters can be used to connect the Angstrom directly tothe microscope objectives for OEM applications. By creating an illumination pathway that is both accessible and optically refined; the microscope objectives for OEM applications. By creating an illumination pathway that is both accessible and optically refined; the Angstrom provides a platform for advanced applications which require precision, resolution, stability, quality light projection, and reproducibility.
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