Morpho Strider

Morpho Strider

Images and results produced in various applications with Morpho Strider

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Morpho Strider is the latest version for General purpose imaging.

It is as user-friendly as before and includes some more advanced features to automate the entire image analysis process and of course includes all the functionality of it’s predecessors : Morpho Lite, Pro & Expert

Data management

1. Flexible organization of the data to suit the experiment scheme through a folder tree structure:

  • Handle large number of images
  • Search images quickly
  • Visualize 5D data-sets
  • Process large numbers of images easily, without programming
  • Display results for complete experiments in integrated Excel module

2. Compatible with many image formats:

  • TIF,
  • OME-TIF,
  • Leica LIF,
  • Zeiss LSM,
  • Metamorph STK,
  • JPEG, BMP…

3. Capable of managing large images, i.e producing stitched images

  • Explora Nova Tiff-based format
  • Aperio SVS
  • Hamamatsu NDPi
  • Zeiss Mirax
  • Leica SCN

 

Acquisition

1. With complete automation capability, Morpho Strider is designed for multi-dimensional acquisition:

  • Multiple wavelengths: capacity to acquire multiple fluorochrome and/or brightfield, phase contrast, DIC images
  • Z-series and automatic creation of extended depth of focus image
  • Time lapse: unlimited number of time-series, link specific time-series with an event
  • Multiple stage positions
  • Stitched image width auto-focus feature
  • Any combination of previous acquisition modes

2. Control the illumination intensity

3. Capable of Structured illumination acquisition which provides “confocal” capabilities to widefield microscopes.

4. Improve the fluorescence image resolution with the Optigrid system and Morpho Strider Opti Acquisition.

Fluorescence

General Image Analysis

1. Processing functionality:

  • Various Color operations
  • Contrast/Luminosity improvement
  • Morphology filters
  • Noise filtering
  • Edge detection
  • Background and shading correction
  • Fast Fourier Transform (FFT) operations
  • Image arithmetic
  • 2D Tracking
  • Batch processing of images.

2. Object extraction:

  • Threshold: automatic, adaptative, magic tool or manual. Set several thresholds to extract several categories of objects simultaneously.
  • Automatic Color classification: users just have to indicate the number of different categories (according to color criterion) and Morpho Strider will do the rest.
  • Spot detection: wavelet-based algorithm to detect small objects. A very robust algorithm, even with noise or heterogeneous background.
  • Supervised classification: Systematically teach the software which objects the user would like to extract. Morpho Strider computes a set of attributes (color, texture, shape…) to identify the objects. Once taught, Morpho Strider will extract all the objects with the same characteristics automatically.

3. Measurement:

For each extracted object, there is the possibility to calculate various parameters:

  • Morphological parameters: Surface – Perimeter – Diameter – Form factor – Elongation factor – Roughness – Length – Angle – Center of gravity – Holes number – Holes surface – Transition number.
  • Intensity-based parameters: Minimum – Maximum – Mean – Sum – Standard deviation – Optical density.

 Fluorescence Image analysis

1. Deconvolution:

– Theoretical Point Spread Function (PSF) computation.

– Noise reduction

– Background correction

– 2D algorithms:

    • No Neighbor
    • Efthia
    • Gold-Meinel
    • Constrained Maximum likelihood
    • Blind

– 3D algorithms:

    • No Neighbor
    • Constrained Maximum likelihood

 

2. Colocalization:

  • Statistic module: scatter plot, correlation coefficients, Mean Deviation Product (MDP)
  • Quantitative module: surface – number – ratio of colocalized probes

 

3. FRET / FRAP

4. Ratio imaging

5. Custom extraction operations

Fluo Cells

Filaments

 

3D reconstruction

– Several methods are available for 3D reconstruction:

  • Texture rendering
  • Ray tracer rendering
  • Iso-surface rendering

– Free handling of the volume

– 4D visualization (3D + time)

– Plane slicing

– Video or image export

– 3D measurement: distance, surface, volume

 

3D Reconstruction

Customization

In consultation with Explora Nova, there is the possibility of including additional functionality to meet the user’s specific needs.